DEAE Sephacel, 500 mL

DEAE-Sephacel Cytiva 17-0500-01, pack of 500 mL GE17-0506-01, Mono Q® HR 1610 GE17-0507-01, Mono S® HR 1610 GE17-0510-01, Q Sepharose® Fast Flow Cytiva 17

Chromatography on DEAE-Sephacel The active fractions of the preceding step were pooled and dialyzed overnight against M potassium phos- phate

deae resin DEAE Weak anion exchanger; Working range, pH 2–9; Binds molecules with predominantly negative charge, repels molecules a predominantly

deae cellulose column chromatography Ion-exchange chromatography of 17* on DEAE Sephacel Inset: FPLC analysis of the end peak from DEAE