Cytiva DEAE-Sephacel Media 500mL:Protein Analysis Reagents

DEAE-Sephacel column at

Workflow of human aorta proteoglycan extraction, isolation by diethylaminoethyl –Sephacel chromatography and analysis LC-MSMS

deae sephacel DEAE Sephacel in the cold The loading ratio is the equivalent of 1 g of wet spleen 1 ml of DEAE Sephacel We typically load the equivalent of 100-125 g

deae resin The supernatant then was clarified by centrifugation at 105,000 x g,, for 60 min and loaded onto a x 40-cm column of DEAE-Sephacel at 24 mlh